Bioluminescence Assays and Reagents
SignaGen Laboratories LV-EF1α-GFP-T2A-fLuc-Puro, Unit Size: 2 x 25 µL (>10^9 TU/mL)
LV-EF1α-GFP-T2A-fLuc-Puro is a pre-made lentivirus that over-expresses eGFP and firefly luciferase (fLuc) via T2A linker under the EF1α promoter with co-expression of puromycin selection in an IRES cassette. Ready to use format.
BIOHIPPO CMV-ACVR1C-RFP-BSD
CMV-ACVR1C-RFP-BSD lentiviral vector expressing RFP under CMV promoter Each vial provides a nominal titer of 2x10E6 transducing units TU Human ACVR2B ORF cDNA Lentivirus Lentiviral particles packaged with expression vector for human ACVR2B (uniprot ID Q8NER5) A CMV promoter drives expression of the ORF fused to a C-terminal epitope tag and fluorescent reporter which is separated by a self-cleaving peptide These lentiviral particles allow for easy transduction and high stable expression of the indicated ORF cDNA in mammalian cells The lentiviral vectors have been optimized to provide high gene expression and reliable genome integration allowing for easy establishment of long-term stable cell lines with consistent read out of fluorescent (GFP RFP BFP) or luminescent (firefly luc) reporters
Abcam IQDMA, Cell-permeable STAT5 inhibitor, 10MG
MW 304.39 g/mol, Purity >98%. Cell-permeable STAT5 inhibitor (IC₅₀ = 8 μM). Indoloquinoline derivative. Antineoplastic agent with broad spectrum antitumor activity seen in vitro. Induces cell-cycle arrest at the G₂/M phase.
The product is subject to the following: Abcam Restricted Use Statement
Abcam D-Luciferin, chemiluminescent luciferase substrate, 10MG
MW 280.3 Da, Purity >98%. Cell-permeable chemiluminescent luciferase substrate. Emits lights upon oxidative decarboxylation in the presence of ATP. Provides a bioluminescent signal for cellular ATP levels in vivo. Water soluble analogs are D-Luciferin potassium salt (ab143655) and D-Luciferin sodium salt (ab145164).
The product is subject to the following: Abcam Restricted Use Statement
Vector Biolabs AP1-Luc Adenovirus (Ad-AP1-Luc)
Expression of the Photinus pyralis (firefly) luciferase gene is controlled by a synthetic promoter that contains direct repeats of the transcription recognition sequences for the activator protein 1 (AP1).When this Luciferase reporter adenovirus and an uncharacterized gene are cotransfected into mammalian cells, the activation of endogenous protein kinases initiated by the cotransfected gene product will result in the activation of corresponding trans-activators which in turn stimulate luciferasae expression.
BIOHIPPO EF1a-KRAS-G12C-V5/GFP
EF1a-KRAS-G12C-V5/GFP lentiviral vector expressing V5 under EF1a promoter Each vial provides a nominal titer of 2x10E6 transducing units TU Human KRAS G12C ORF cDNA lentivirus Lentiviral particles packaged with expression vector for G12C mutant of human KRAS proto-oncogene GTPase (KRAS) transcript variant a An EF1 promoter drives expression of KRAS G12C fused to a C-terminal V5 tag and a GFP reporter which is separated from the ORF cDNA by a self-cleaving peptide These lentiviral particles allow for easy transduction and high stable expression of the indicated ORF cDNA in mammalian cells The lentiviral vectors have been optimized to provide high gene expression and reliable genome integration allowing for easy establishment of long-term stable cell lines with consistent read out of fluorescent (GFP RFP) or luminescent (firefly luc) reporters
Biotium Coelenterazine 400A (DEEPBLUEC, A TRADEM
Coelenterazine 400a, also called DeepBlueC™, is a coelenterazine derivative that serves as a substrate for Renilla luciferase (Rluc) and generates an emission peak centered around 400 nm. It is the preferred Rluc substrate for BRET studies because it has minimal interference with the emission of the GFP acceptor. Properties : Off-white to pinkish solid soluble in MeOH or EtOH; Store at -20°C, protect from light; C26H21N3O; MW: 391.46. DeepBlueC is a trademark of BioSignal Packard.
American Research Products Inc LUCIFERASE REPORTER GENE KIT B
5000265456 LUCIFERASE REPORTER GENE KIT B
BPS Bioscience Inc SBE Luciferase Reporter Lentivirus, 2 vials, 500 μl each, TGFβ/SMAD signaling pathway, Firefly Luciferase, ≥5 x 10^6 TU/ml, -80°C storage
The SBE Luciferase Reporter Lentivirus is engineered to facilitate the monitoring of the TGFβ/SMAD signaling pathway. This replication-incompetent lentiviral particle, utilizing HIV-based and VSV-G pseudotyping technology, incorporates a firefly luciferase gene regulated by a multimerized SBE-responsive element. It offers a sensitive method for detecting changes in the TGFβ/SMAD pathway activity in mammalian cells. This product serves as an essential tool for those aiming to screen potential pathway modulators and develop stable reporter cell lines.
Gold Biotechnology Inc 100mg
Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.